Ponies Play Hotel Remorse is a video posted in April 2016 by BronyGuardian. It mostly depicts the main characters of Friendship Is Magic playing through the game Hotel Remorse (which already has a few jumpscares in it). The actual screamer comes by the end of the video.
It is another screamer that only appears at a certain time in the video.
The screamer itself appears directly after each character finishes the game (and getting freaked out by the finale scare), right after the video thanks you for watching. What follows is a flurry of creepy fan art of the show (including Zalgo styled images) while a man roaring plays in the background. The video then ends abruptly.
WARNING: The following video contains a screamer (as well as in-game scares).